Dr. Ruth Wilkins

Adjunct Research Professor
Ionizing Radiation Health Sciences Division, - Consumer and Clinical Radiation Protection Bureau - Health Canada
775 Brookfield Road, PL 6303B,
Ottawa, Ontario K1A 1C1
(613) 941 - 7263
ruth [dot] wilkinsathc-sc [dot] gc [dot] ca

 Research Summary

My laboratory at Health Canada is focusing on the biological effects of ionizing radiation.  The main research project is focused on biological dosimetry which is a method of measuring the amount of ionizing radiation received by an individual using biological material.  This type of dosimeter is essential when an individual is accidentally exposed and no physical dosimetry is available. Currently, the accepted method of biological dosimetry is the dicentric chromosome assay which involves examining chromosomes for characteristic damage caused by ionizing radiation. This is a very tedious and time consuming assay, requiring weeks to process a sample from one individual. For large scale radiological events where thousands of individuals might be exposed to ionizing radiation, a biological dosimeter is desirable that could analyse many samples in a timely manner. Our laboratory is currently exploring new techniques for biological dosimetry. 

 Selected Publications  

  1. Beaton-Green LA, Rodrigues MA, Lachapelle S, Wilkins RC, Foundations of identifying individual chromosomes by imaging flow cytometry with applications in radiation biodosimetry. Methods 112: 18-24; 2017.

  2. Brzozowska B, Ainsbury E, Baert A, Beaton-Green L, Barrios L, Barquinero JF, Bassinet C, Beinke C, Benedek A, Beukes P, Bortolin E, Buraczewska I, Burbidge C, De AA, De AC, Della MS, Depuydt J, De SS, Dobos K, Domene MM, Dominguez I, Facco E, Fattibene P, Frenzel M, Monteiro GO, Gonon G, Gregoire E, Gruel G, Hadjidekova V, Hatzi VI, Hristova R, Jaworska A, Kis E, Kowalska M, Kulka U, Lista F, Lumniczky K, Martinez-Lopez W, Meschini R, Moertl S, Moquet J, Noditi M, Oestreicher U, Orta Vazquez ML, Palma V, Pantelias G, Montoro PA, Patrono C, Piqueret-Stephan L, Quattrini MC, Regalbuto E, Ricoul M, Roch-Lefevre S, Roy L, Sabatier L, Sarchiapone L, Sebastia N, Sommer S, Sun M, Suto Y, Terzoudi G, Trompier F, Vral A, Wilkins R, Zafiropoulos D, Wieser A, Woda C, Wojcik A, RENEB accident simulation exercise. Int J Radiat Biol 93: 75-80; 2017.

  3. Chauhan V, Duncan D, Wilkins RC, Radiological/Nuclear Human Monitoring Tabletop Exercise: Recommendations and Lessons Identified. Health Phys 112: 580-586; 2017.
  4. Citrin DE, Prasanna PGS, Walker AJ, Freeman ML, Eke I, Barcellos-Hoff MH, Arankalayil MJ, Cohen EP, Wilkins RC, Ahmed MM, Anscher MS, Movsas B, Buchsbaum JC, Mendonca MS, Wynn TA, Coleman CN, Radiation-Induced Fibrosis: Mechanisms and Opportunities to Mitigate. Report of an NCI Workshop, September 19, 2016. Radiat Res 188: 1-20; 2017.
  5. Oestreicher U, Samaga D, Ainsbury E, Antunes AC, Baeyens A, Barrios L, Beinke C, Beukes P, Blakely WF, Cucu A, De AA, Depuydt J, De SS, Di GM, Dobos K, Dominguez I, Duy PN, Espinoza ME, Flegal FN, Figel M, Garcia O, Monteiro GO, Gregoire E, Guerrero-Carbajal C, Guclu I, Hadjidekova V, Hande P, Kulka U, Lemon J, Lindholm C, Lista F, Lumniczky K, Martinez-Lopez W, Maznyk N, Meschini R, M'Kacher R, Montoro A, Moquet J, Moreno M, Noditi M, Pajic J, Radl A, Ricoul M, Romm H, Roy L, Sabatier L, Sebastia N, Slabbert J, Sommer S, Stuck OM, Subramanian U, Suto Y, Que T, Testa A, Terzoudi G, Vral A, Wilkins R, Yanti L, Zafiropoulos D, Wojcik A, RENEB intercomparisons applying the conventional Dicentric Chromosome Assay (DCA). Int J Radiat Biol 93: 20-29; 2017.
  6. Wilkins RC, Rodrigues MA, Beaton-Green LA, The Application of Imaging Flow Cytometry to High-Throughput Biodosimetry. Genome Integrity 8: 7-2017.
  7. Beaton-Green LA, Barr T, Ainsbury EA, Wilkins RC, Retrospective Biodosimetry of an Occupational Overexposure-Case Study. Radiat Prot Dosimetry 172: 254-259; 2016.
  8. Beaton-Green LA, Wilkins RC, Quantitation of Chromosome Damage by Imaging Flow Cytometry. Methods Mol Biol 1389: 97-110; 2016.
  9. Chauhan V, Kuo B, McNamee JP, Wilkins RC, Yauk CL, Transcriptional benchmark dose modeling: Exploring how advances in chemical risk assessment may be applied to the radiation field. Environ Mol Mutagen 57: 589-604; 2016.
  10. Li Y, Knoll JH, Wilkins RC, Flegal FN, Rogan PK, Automated discrimination of dicentric and monocentric chromosomes by machine learning-based image processing. Microsc Res Tech 79: 393-402; 2016.
  11. Rodrigues MA, Beaton-Green LA, Wilkins RC, Validation of the Cytokinesis-block Micronucleus Assay Using Imaging Flow Cytometry for High Throughput Radiation Biodosimetry. Health Phys 110: 29-36; 2016.
  12. Rodrigues MA, Probst CE, Beaton-Green LA, Wilkins RC, The effect of an optimized imaging flow cytometry analysis template on sample throughput in the reduced culture cytokinesis-block micronucleus assay. Radiat Prot Dosimetry 172: 223-229; 2016.
  13. Rodrigues MA, Probst CE, Beaton-Green LA, Wilkins RC, Optimized Automated Data Analysis for the Cytokinesis-Block Micronucleus Assay Using Imaging Flow Cytometry for High Throughput Radiation Biodosimetry. Cytometry A 89: 653-662; 2016.
  14. Rogan PK, Li Y, Wilkins R, Flegal FN, Knoll JH, Radiation Dose Estimation by Automated Cytogenetic Biodosimetry. Radiat Prot Dosimetry 172: 207-217; 2016.
  15. Romm H, Beinke C, Garcia O, Di GM, Gregoire E, Livingston G, Lloyd D, Martinez-Lopez W, Moquet JE, Sugarman SL, Wilkins RC, Ainsbury EA, A New Cytogenetic Biodosimetry Image Repository for the Dicentric Assay. Radiat Prot Dosimetry 172: 192-200; 2016.
  16. Wilkins RC, Carr Z, Lloyd DC, An update of the WHO Biodosenet: Developments since its Inception. Radiat Prot Dosimetry 172: 47-57; 2016.
  17. Beaton-Green LA, Lachapelle S, Straube U, Wilkins RC, Evolution of the Health Canada astronaut biodosimetry program with a view toward international harmonization. Mutat Res Genet Toxicol Environ Mutagen 793: 101-106; 2015.
  18. Wilkins RC, Beaton-Green LA, Lachapelle S, Kutzner BC, Ferrarotto C, Chauhan V, Marro L, Livingston GK, Boulay GH, Flegal FN, Evaluation of the annual Canadian biodosimetry network intercomparisons. Int J Radiat Biol 91: 443-451; 2015.