Matt Rodrigues and Dan La Russa

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Thursday, October 24, 2013
3:30 pm
Room RPB 205B (boardroom), Health Canada, 775 Brookfield Road

1. "An automated high-throughput method of the cytokinesis block micronucleus (CBMN) assay for dose estimation in radiation biodosimetry"
Matthew Rodrigues - Carleton University

Abstract: The cytokinesis-block micronucleus (CBMN) assay is employed in biological dosimetry as a method for determining the dose of radiation to an exposed individual from the frequency of micronuclei (MN) in binucleated lymphocyte cells. The assay is typically performed using manual microscopy but it would be advantageous to automate the method to allow for increased throughput. With the development of new technologies such as the ImageStreamX, an imaging flow cytometer, it is now possible to adapt the CBMN assay to an automated imaging cytometry method. The ImageStreamX has adequate sensitivity to quantify radiation doses to within ~0.5 Gy while adding the increased throughput of traditional flow cytometry. The protocol and analysis which adapts the CBMN assay for use on the ImageStreamX will be presented as well as recent results which indicate that binucleated cells (BNCs) and MN can be identified, imaged and enumerated automatically using the ImageStreamX, allowing for dose estimation.

2. "Quality and safety initiatives in radiation therapy at the Ottawa Hospital Cancer Centre"
Daniel La Russa - The Ottawa Hospital Cancer Centre

Abstract: This presentation will review some of the recent updates to the quality management of the Radiation Medicine Program at The Ottawa Hospital Cancer Centre (TOHCC). Emphasis will be put on the use of Failure Mode and Effect Analysis (FMEA) and the use of Statistical Process Control (SPC) in the context of a modern radiation therapy treatment process. Examples of the use of these techniques at TOHCC will be presented along with a overview of some device-centric quality control tests of our IMRT/VMAT treatment processes. A project underway to develop free, open source software for quality control of radiation therapy treatment plans will also be described.